January Meeting Announcement, Delaware Valley Mass Spectrometry Discussion Group

• Topic: "Screening of Proteins from Cancer Cells Using 2-D Liquid Separations and CE/Tandem Mass Spectrometry"

• Speaker: David M. Lubman, The University of Michigan

• Date: Monday, January 10, 2000. 7:00 PM

• Time: Social Hour: 7:00 PM.
  Talk: 7:30 PM.

• Place: Merck, West Point, 37 Auditorium

• Abstract: A 2-D liquid phase separation procedure has been developed for separation of proteins from cellular extracts. This method depends upon the use of isoelectric focusing in the liquid phase followed by RPHPLC using nonporous-coated silica bead-packed columns. This method is capable of separating hundreds of proteins from cellular extracts and of providing quantitative information on the relative change in protein expression. The 2-D liquid method provides information on the protein content of the cell analogous to that of 2-D Gel electrophoresis and can provide isolated proteins in the liquid phase for further analysis by mass spectrometry. Capillary electrophoresis/time-of-flight mass spectrometry (CE-TOFMS) has been used for detailed analysis of proteins isolated by this method using peptide mapping and on-line MS/MS of protein digests. The method will be demonstrated for analysis of key proteins isolated from esophageal adenocarcinoma and breast cancer cells, where modified versions of known proteins often serve as important markers in the progression oftumorogenesis or metastasis.

• Bio: David M. Lubman received his A.B. from Cornell in 1975 and his Ph.D. fromStanford in 1979 in Physical Chemistry under the direction of Professor Richard N. Zare. He worked as a staff scientist for Quanta-Ray in Palo Alto from 1979-1983 and was a Senior Fellow at the Weizmann Institute in Rehovot, Israel during 1982-1983. He is currently Professor of Chemistry at the University of Michigan. His current interests are in biotechnology, bioanalytical chemistry, separations/mass spectrometry, proteomics and cancer biology.

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