October 2000 Meeting Announcement, Delaware Valley Mass Spectrometry Discussion Group
- Topic: "MALDI Mass Spectrometry of Nonpolar Compounds: New Techniques and Tips"
- Speaker: Patrick A. Limbach, Louisiana State University
- Date: Monday, October 9, 2000. 7:00 PM
- Time: Social Hour: 7:00 PM.
Talk: 7:30 PM.
- Place: Merck, West Point, 37 Auditorium
- Abstract: With the recent development of electrospray ionization (ESI) and matrix-assisted laser desorption/ionization (MALDI), mass spectrometry has become a powerful analytical tool for the characterization of polymers and biopolymers. However, the majority of compounds which are amenable to these two ionization methods are polar. During this presentation, I will cover our research into expanding MALDI mass spectrometry into the area of nonpolar compound analysis. The first part of this talk will cover our use of surfactants to actually improve the coverage of tryptic peptides during peptide mapping experiments. Surfactants such as SDS, when added in high enough concentrations, improve our ability to detect hydrophobic peptides present in digests of various proteins and are especially useful for the analysis of membrane proteins. The second part of this talk will focus on our development of nonpolar matrices for charge-transfer ionization and hydrocarbon polymer analysis. Nonpolar matrices provide an alternative ionization pathway for the analysis of low molecular weight nonpolar compounds. In addition, we have found that nonpolar matrices, such as anthracene, are effective alternatives to acidic matrices for the characterization of hydrocarbon polymers. Interestingly, the interaction of the metal salt typically added as a cationization reagent is dramatically different with nonpolar matrices as compared to acidic matrices.
- Bio: Pat received his B.S. in Chemistry and Chemical Physics from Centre College in Danville, Kentucky in 1988. He received his Ph.D. in Analytical Chemistry from The Ohio State University under the direction of Prof. Alan G. Marshall in 1992. He spent two years as a postdoctoral fellow performing research in Nucleic Acid Chemistry with Prof. James A. McCloskey at the University of Utah. In 1995, Pat started as an Assistant Professor at Louisiana State University. He was promoted to Associate Professor in 1999. While at LSU, Pat has received the American Society for Mass Spectrometry Research Award in 1998. Effective June, 2001, Pat will become the Rieveschl Associate Professor of Chemistry at the University of Cincinnati. His research interests include: development of new instrumentation, methodologies and applications of mass spectrometry for the structural characterization and sequence determination of biomolecules with an emphasis on nucleic acids, proteins and lipids; investigations into the role of modified nucleosides in peptide bond formation in the ribosome; development of polymer-based micromachined devices for mass spectrometry; fundamental studies of matrix-assisted laser desorption/ionization mass spectrometry; and development of new methodologies and applications of mass spectrometry for the characterization of nonpolar technical polymers.
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