September 2001 Meeting Announcement, Delaware Valley Mass Spectrometry Discussion Group
- Topic: "FT-ICR MS of Proteins"
- Speaker: Susan Martin University of Sciences in Philadelphia
- Date: Monday, September 10, 2001. 6:30 PM
- Time: Social Hour: 6:30 PM. (Pizza and Beer)
Talk: 7:30 PM.
- Place: Widener University, Webb Room.
- Abstract: FT-ICR MS is a powerful tool for protein identification due to its
excellent mass accuracy, ability to perform multiple stage tandem mass spectrometry (MSn) experiments and high sensitivity. FT-ICR MS has become invaluable for analysis of large biomolecules such as intact proteins. Recent advances in FT-ICR MS technology have demonstrated that FT-ICR has the potential to be an effective mass spectrometric tool for the analysis
of small proteins and peptides as well. Using FT-ICR mass spectrometry protein identification can be achieved based on both peptide mass mapping and amino acid sequence comparisons. Peptide ions may be individually analyzed when they are present within a mixture of peptides. The ultra-high mass accuracy improves confidence in protein assignments and decreases search times using computer database searching algorithms. The FT-ICR mass spectrometer at the University of Virginia is equipped with variable flow n-HPLC with mESI and was used to analyze peptides at the sub-femtomole level. Mass mapping was demonstrated for a standard protein
present at 250 attomoles. A mixture of six known proteins present in a 1000-fold concentration range was digested with trypsin. Predicted peptide fragments were identified from the mixture. Photodissociation of some of the peptide ions generated spectra from which amino acid sequence
information could be obtained. Using sequence comparisons, the protein identities were retrieved from a sequence database. Finally, a mixture of unknown proteins was mass-analyzed using photodissociation to generate partial primary structure information. Four proteins were identified from sequence databases by means of amino acid sequence comparison.
Susan E. Martin
1993: Received my B.A. in Chemistry from Hanover College, (Hanover, IN)
1999: Received my PhD in Chemistry from the University of Virginia
· Research Advisor: Professor Donald F. Hunt
· Area of Specialization: Analytical Chemistry, Biological Mass
1999- Visiting Assistant Professor and Post Doctoral Research Associate
2001 Furman University (Greenville, SC)
· Advisor: Professor John F. Wheeler
· Area of Research: Separations, Capillary Electrophoresis, Environmental
Presently teaching at the University of Sciences in Philadelphia
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