September 2002 Meeting Announcement, Delaware Valley Mass Spectrometry Discussion Group
- Topic: "Surface Plasmon Resonance - Mass Spectrometry"
- Speaker: Jack Simpson, Protein Chemistry Laboratory, SAIC-NCI-Frederick
- Date: Monday, September 9, 2002. 6:30 PM
- Time: Social Hour: 6:30 PM. (Pizza and Beer)
Talk: 7:30 PM.
- Place: Widener University, Webb Room.
- Abstract: The characterization of protein interactions: protein-protein, protein-DNA or protein-ligand, is central to the elucidation of the role of proteins in vivo. A key technique for studying these interactions is surface plasmon resonance spectroscopy (SPR). Using SPR, kinetic studies can be performed on the binding of analytes to targets bound to a chip surface. While SPR is exquisitely sensitive, the detection of binding partners is non-specific. To address this concern, and to take advantage the inherent affinity capture abilities of the SPR chips used, several groups have focused on combining SPR with mass spectrometry. The purpose of this discussion will be an introduction to the principles of SPR and recent advances in interfacing SPR with mass spectrometry. In addition, our recent work combining MALDI with SPR for the characterization of HIV nucleocapsid protein (NCp7) will be presented.
Jack Simpson received his B.S. degree in Chemistry from Louisiana State University, a M.S. degree in Organic Chemistry from Texas A&M University, and a Ph.D. in Analytical Chemistry from American University, while working with Sandy Markey at the National Institutes of Health. His career as a chemist has centered on the application of bioanalytical techniques to problems in chemistry, biochemistry and medicine.
Jack Simpson has worked at Bristol-Myers Squibb, the National Institutes of Health, the U.S. Food and Drug Administration and PharmaKinetics (a contract lab). He recently joined the Protein Chemistry Laboratory at the NCI in Frederick, Maryland after directing a genetics laboratory at the Armed Forces Institute of Pathology. He is, like many others, trying to rapidly become a protein chemist.
In his spare time, he teaches the Japanese martial art of Aikido, and can whip up a mean pot of Gumbo.
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