March 2004 Meeting Announcement, Delaware Valley Mass Spectrometry Discussion Group

• Topic: "Comparative Proteomics: When can LCMS Contribute? "

• Speaker:Catherine C. Fenselau, Professor, University of Maryland.

• Date: Tuesday, March 2, 2004. 6:30 PM

• Time: Social Hour: 6:30 PM. (Pizza)
  Talk: 7:30 PM.

• Place: Fox Chase Cancer Center. Reimann Auditorium.

• Directions:Park in the North parking lots along the North Drive (please see the Campus map at http://www.fccc.edu/information/campus_map.html) and enter the Reimann Building at its front entrance beside the circle. Once inside, you will be directed to the appropriate venue. For directions and additional maps see http://www.fccc.edu/information/directions.html

• Abstract: We are studying changes in protein abundances in human breast cancer cells that have become resistant to anticancer drugs. This requires quantitative comparisons of protein levels in drug susceptible and drug resistant cell lines. We have employed three approaches to this quantitative comparison. In one we use computer-supported densitometry comparisons of two-dimensional gel arrays. In the other two, isotope ratios of peptide pairs are measured by mass spectrometry. In one case heavy or light labeled amino acids are introduced metabolically during cell culture (also called SILAC) and proteins are separated by two dimensional gel electrophoresis. Proteins are excised and digested to peptides for final analysis by electrospray or MALDI mass spectrometry. In the last type of experiment, O-18 and O-16 are introduced by proteolytic labeling into the carboxyl termini of peptides produced from proteins from two different cell lines (Yao, et al, Anal.Chem. 2001). This last strategy is compatible with "shot-gun" strategies that rely heavily on LC-MSMS or LC-LC-MSMS. Results will be presented obtained by applying each of these three methods to the cytosolic fraction of MCF-7 cells, and some comments will be offered on the relative merits of the three.

• Bio:Catherine Fenselau is Professor of Chemistry and Biochemistry at the University of Maryland, and a member of the Greenebaum Cancer Center in the University of Maryland Medical Center. Her research interests include drug/protein interactions, mechanisms of acquired drug resistance, new methods for proteomics, and rapid detection of airborne microorganisms. She has published more than 300 peer-reviewed papers and trained more than 100 graduate students and post-doctoral fellows. She is past president of the American Society for Mass Spectrometry, a member of the founding council of the International Society for Human Proteomics, past chair of the ACS Division of Analytical Chemistry, founding editor of Biomedical Mass Spectrometry (now J. Mass Spectrometry) and currently an associate editor for the ACS journal Analytical Chemistry. She has received the EAS Award for Achievements in Analytical Chemistry, the AnaChem Award, the Medal of the Spectroscopy Society of Pittsburgh and the Garvan Medal of the American Chemical Society. She is a member of the Board of Trustees of the Maryland Science Center.

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