October 2006 Meeting Announcement, Delaware Valley Mass Spectrometry Discussion Group
- Topic: "Alternative Strategies for Metabolite Identification using the Advion Nanomate and the Sciex Q-Trap
- Speaker: : Richard King, Merck
- Date: Monday, October 9, 2006. 6:30 PM
- Time: Social Hour: 6:30 PM.
Talk: 7:30 PM.
Please RSVP to Christopher Petucci PETUCCC@wyeth.com by Thursday October 5th.
- Place: Department of Chemistry, Villanova University (Room 154, Mendel Hall)
In most cases, on line LC-MS is the method of choice for metabolite identification. Many different mass analyzers and approaches are widely used with success. The advantage offered by the Q-Trap is the variety of scans available using the triple quadrupole, the linear ion trap and the hybrid quad-trap combination scans. The Nanomate from Advion also has some advantages over traditional on-line LC-electrospray MS. In particular, the low sample consumption rates and the excellent sensitivity of the nano-electrospray offer advantages when coupled with off-line separation. The workflow was first published by Hopfgartner et al. We have found this workflow particularly useful for identifying metabolites from radiometric profiles collected as fractions from an HPLC separation. The radioactive fractions can be interrogated thoroughly using the infusion nano-electrospray and the variety of scans available on the Q-trap. The presentation will focus on the advantages offered by the Q trap and the Nanomate and on the value of the work flow described for two types of studies as examples, metabolite profiling from tissue slices made for QWBA and metabolite profiling from plasma taken from animals dosed with radio labeled drug.
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