Several commercially available drug compounds were analyzed by LC/UV/ESI and DART interfaced to a Waters quadrupole MS. The mass spectra obtained by DART/MS for the molecular ions of these compounds agrees with that obtained by LC/UV/ESI-MS. The main difference between these techniques was that a few dozen compounds could be analyzed by DART/MS within 10 minutes while the LC/UV/ESI-MS experiments took more than 1.5 hours.
DART/MS was also examined to determine if it could be used to monitor synthetic organic reactions in drug discovery and for the molecular weight confirmation of final products. For simple reaction monitoring, the trends in mass spectral signals for the reactant and product obtained using DART/MS scale with those of the diode array or the total ion chromatogram obtained by LC/UV/ESI-MS. However, complex reaction mixtures could not be successfully monitored using DART/MS. In general, DART is a new ionization tool to complement LC/UV/ESI-MS for the rapid characterization of compounds in drug discovery.
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