This presentation will focus on the routine use of UHPLC systems coupled to tandem mass spectrometers for the analysis of analytes in biological matrices. Analysis times have been decreased to 1.1 minutes while sample resolution has been maintained. Data will be presented on the coupling of an Acquity UPLC system to an API4000 mass spectrometer along with the interfacing of an Agilent 1200 UHPLC system to an API4000. This presentation will focus on the ease of use for the various UHPLC systems and their advantages over a standard HPLC system. Considerations of increased sensitivity, linear range, effect of dwell time and the use of ESI and APCI will also be presented.
Since 2004, Dr Morgan has lead the Bioanalytical Research Group at Bristol-Myers Squibb at the Wallingford CT site. The group is responsible for providing quantitative LC/MS/MS data for discovery programs. His research interests include: effects of ion suppression in ESI and coupling of supercritical fluid chromatography with mass spectrometry.
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