September 2010 Meeting Announcement, Delaware Valley Mass Spectrometry Discussion Group
PLEASE NOTE: We will meet in Mendel 154.
- Topic: "Single Cell Analysis Using LAESI and NAPA Mass Spectrometry"
- Speaker:Akos Vertes, The George Washington University
- Date: Monday, September 13, 2010. 6:30 PM
- Time: Social Hour: 6:30 PM.
Talk: 7:30 PM.
Please RSVP to Karen WendlingWendlingK@chc.edu by Thursday September 9th.
- Place: Department of Chemistry, Villanova University (Room 154, Mendel Hall)
Seemingly uniform populations of a given cell type exhibit heterogeneity in their biomolecular composition. These differences can stem from genetic variations, but they can also be generated by the cell cycle, environmental factors, or disease states. Cellular heterogeneity is linked to drug resistance in infections and to low responders in the treatment of cancer. Analyzing single cells is a challenge because of their small volume and molecular complexity. Whereas genomic and proteomic analysis can be rendered by microarray technology and by mass spectrometry, respectively, the metabolic make-up of single cells is currently less accessible. We introduced two new ionization methods that enabled the identification of diverse metabolites directly from single cells. Laser ablation electrospray ionization (LAESI) is capable of detecting over 300 ionic species from large single cells . Silicon nanopost arrays (NAPA), the first example of nanophotonic ion sources, can detect ~20% of the metabolites in the known yeast metabolome, and has the ability to analyze a single yeast cell . This presentation focuses on applications of the LAESI and NAPA ionization methods to problems of biological relevance.
1. B. Shrestha and A. Vertes, "In Situ Metabolic Profiling of Single Cells by Laser Ablation Electrospray Ionization Mass Spectrometry," Anal. Chem., 2009, 81, 8265.
2. B. N. Walker, J. A. Stolee, D. L. Pickel, S. T. Retterer and A. Vertes, "Tailored Silicon Nanopost Arrays for Resonant Nanophotonic Ion Production," J. Phys. Chem. C, 2010, 114, 4835.
Akos Vertes is a Professor of Chemistry and a Professor of Biochemistry and Molecular Biology at the George Washington University in Washington, DC. He is a co-founder and co-director of the W. M. Keck Institute for Proteomics Technology and Applications, a center of strategic excellence at the university. His research interests span from fundamental studies in analytical and physical chemistry to the development of new technologies for biomedical analysis. Recent accomplishments include the introduction of photonic ion sources based on nanostructures, the introduction of a new ambient ion source called laser ablation electrospray ionization (LAESI), metabolic imaging of biological tissue under native conditions and the in situ mass spectrometric analysis of single cells. His research has been presented in over 120 peer-reviewed publications, in two books, the first on laser ionization and the second on the medical applications of mass spectrometry, which he co-edited and co-authored, and in numerous conference presentations. His honors and awards include serving as a member of the Committee of Visitors reviewing the National Science Foundation, Division of Chemistry, delivering numerous distinguished lectures including the Velmer A. Fassel Lecture in Analytical Chemistry, receiving the Oscar and Shoshana Trachtenberg Prize for Scholarship and the Elsevier/Spectrochimica Acta Award, becoming the Fellow of the Royal Flemish Academy for Science and the Arts in Brussels, Belgium, and the Doctor of the Hungarian Academy of Sciences in Budapest, Hungary. He is a member of the News & Features Advisory Panel for the journal Analytical Chemistry.
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