November 2010 Meeting Announcement, Delaware Valley Mass Spectrometry Discussion Group
PLEASE NOTE: We will meet in Mendel 154.
- Topic: "Emerging Clincal Applications of Mass Spectrometry: The MALDI Biotyper for Identification and Classification of Microorganisms; MALDI Imaging for Biomarker Discovery and Small Molecule Imaging"
- Speaker: Gary Kruppa, Bruker Daltonics
- Date: Monday, November 8, 2010. 6:30 PM
- Time: Social Hour: 6:30 PM.
Talk: 7:30 PM.
Please RSVP to Karen Wendling WendlingK@chc.edu by Thursday November 4th.
- Place: Department of Chemistry, Villanova University (Room 154, Mendel Hall)
Two applications of MALDI-TOF mass spectrometry with clinical applications will be discussed. Rapid and reliable identification of microorganisms is an essential requirement, e.g. in the pharmaceutical industry, food quality control, environmental analysis, and veterinary and medical healthcare. Currently, methods based on morphological investigation and tests based on the biochemistry of the microorganisms are most routinely used. Unfortunately, these methods have limitations in resolution, applicability and reliability. Frequently, they end up in genus determination. Modern molecular methods which have a higher resolution power are generally too laborious and costly for high-throughput routine usage, and in clinical diagnostics the time required for some of these methods may have a negative impact on treatment outcomes. MALDI-TOF mass spectrometry fingerprinting has been shown to be well suited for the identification of microorganisms. The utilisation of this technology in combination with a dedicated bioinformatic software, the MALDI Biotyper 2.0 for the rapid, reliable detection of clinically relevant bacteria and fungi on the species as well as the subspecies level will be reported.
MALDI-MS imaging of proteins and peptides in tissues provides pictures of molecular distributions in tissues that can be examined together with optical images from tissue stained with dyes used in traditional histology for an approach that may be termed "molecular histology". A complete solution for MALDI matrix application to the tissue, imaging data acquisition, and statistical and other software tools for producing useful images from the data will be presented. The use of molecular histology for the classification of tissue health state, and for biomarker discovery will be discussed. Small molecule imaging provides access to images of lipids, metabolites and other endogenous small molecules that may also serve as biomarkers for disease states, and is in many ways complementary to peptide and protein imaging. Another area of great interest in small molecule imaging is xenobiotic imaging. Direct imaging of drugs in tissues can demonstrate that the drug has reached the targeted regions of the tissue. Imaging of drug metabolites can yield a direct measurement of the DMPK properties of the drug directly in the tissue. Solutions for small molecule and drug imaging in tissues will be discussed, including direct imaging of drugs in tissues from animals dosed at therapeutic levels using FTMS.
Gary Kruppa is currently Vice President for Business Development at Bruker Daltonics in Billerica Ma. His undegraduatedegree is from the University of Delaware where he conducted undergraduate research with Prof. Douglas Ridge, and one summer at Cornell with Prof. Fred McLafferty. His graduate work was at the California Institute of Technology where he completed his Ph. D. with Jack Beauchamp conducting research in photoelectron spectroscopty and FT-ICR mass spectrometry applied to the energetics of free radicals and their corresponding ions. After that Gary completed Post-Docs at the University of Basel and Ciba Geigy in Basel. He has spent 18 years at Bruker Daltonics where he is currently vice president for business development, and from 2001 to 2004 ran his own research program in FT-ICR mass spectrometry at Sandia National Laboratories.
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