Two recent examples of the use of MS technology in our laboratory will be presented. The first involves the use of iTRAQ peptide labeling for quantitation of MACS2 variants from human mitochondrial extract. MACS2 (medium chain acyl CoA synthetase) is an enzyme involved in ligation of medium chain fatty acids with coenzyme A and is a putative target for metabolic disease. There are three forms of MACS2; two forms of type 2A which differ by a single aa substitution and one form of type 2B which differs by 14 aa substitutions over a 64kDa, 577 aa protein. To quantitate these three forms from human tissue, we utilized unique signature peptides of each form and analyzed using MRM analysis of iTRAQ labeled peptides.
The second example involves the use of the Biocius RapidFire MS inlet system for in-vitro ADME support. The RapidFire device allows MS analysis of samples directly from 96 or 384 well microtiter plates every 10 seconds. The unit was originally designed for high throughput screening of compounds for those assays that require MS detection of enzymatic products. It has also been shown useful for in-vitro and in-vivo ADME support. Some recent data from our research efforts demonstrating its utility in these areas will be presented.
This page has been accessed
times since 9/15 /96 .
Last Updated 02/8/2011