February 2013 Meeting Announcement, Delaware Valley Mass Spectrometry Discussion Group
PLEASE NOTE: We will meet in Mendel 154.
- Topic: "Metabolomic Profiling of Food by LC/MS for Authenticity, Adulteration, and Contamination "
- Speaker:Jerry Zweigenbaum, Agilent
- Date: Monday, February 11, 2013. 6:30 PM
- Time: Social Hour: 6:30 PM.
Talk: 7:30 PM.
Please RSVP to Sergio Nanita firstname.lastname@example.org by Thursday February 7.
- Place: Department of Chemistry, Villanova University (Room 154, Mendel Hall)
Identification of Unknown Toxicants in Fruits and Vegetables Using LC/QTOF MS
The identification of unknown toxic compounds in food is of continuous concern. This presentation discusses the simulation of the occurrence of toxic compounds in different foods and examines a workflow that includes QuEChERS sample preparation, analysis by LC/QTOF MS, statistical evaluation of the data, and identification of suspect compounds. Apple, orange and spinach samples were extracted using the AOAC QuEChERS approach. The extracts were split and one half spiked with compounds known to be toxic but not in either a personal compound pesticide database containing 1600 entries or a forensic toxicology database containing over 7000 entries. Samples were analyzed as either good (without the toxic compounds) or bad (containing the toxins). The LC/MS data was aligned, filtered by frequency, and then analyzed by ANOVA and fold change. The resulting list of masses found only in the “bad” was then used to reanalyze those samples by LC/MS/MS. The workflow to identify these compounds using accurate mass MS and MS/MS is described.
Jerry started working with Agilent in the spring of 2002. He came with a variety of analytical experience spanning 25 years from the beginning of his career as a forensic chemist to the research and development labs at Eastman Kodak. Jerry managed an organic environmental testing lab for Eastman Kodak doing GC/MS, GC/ECD and Hall detection, and LC for environmental pollutants and then a health and safety laboratory. He then worked as a research chemist at the Eastman Kodak Research Labs on Lake Avenue, Rochester, NY. His academic training includes a BS in chemistry, an MS in analytical chemistry, and a PhD in environmental toxicology under the mentorship of professor Jack Henion at Cornell University. He has specialized in LC/MS at Agilent working in the applied markets of industrial chemicals, environmental analysis, forensic, and food safety. He has worked with leading scientists worldwide from Pennsylvania, USA (ethoxylated surfactants), to Almeria, Spain (screening pesticides), to Melbourne, Australia (explosives in the environment), to Beijing, China (Sudan dyes in salty egg), and to York, UK (migrants in packaging material) amongst many others. He focused on food safety many of those years culminating in editing a book titled "Mass Spectrometry in Food Safety" published in 2011.
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