February 2015 Meeting Announcement, Delaware Valley Mass Spectrometry Discussion Group
Please note room change to Mendel Hall 115
- Topic: "Chemoplexing: LC-MS Quantification for Systems Biology"
- Speaker: Carmen Fernández-Metzler, Bonnie Baker, Victoria King and Richard C. King
PharmaCadence Analytical Services, LLC, Hatfield, PA
- Date: Monday, February 9, 2015. 6:00 PM
- Time: Social Hour: 6:00 PM.
Talk: 7:00 PM.
Please RSVP to John Masucci JMasucci@its.jnj.com by Thursday February 5.
- Place: Department of Chemistry, Villanova University (Room 115, Mendel Hall)
- Abstract: Personalized medicine and systems biology require information from a variety of analyses to generate a more complete picture of the entire biological system. This might be thought of simplistically as having two solutions. One is integrative analysis where data from a collection of disparate experiments is combined using informatics and modelling to generate the system-wide picture. The other is a unified approach where traditionally separate analyses are combined into a single analytical event which yields information on the entire system under study. The application of existing technologies already allows us to investigate the unified approach for studying enzyme systems. By developing methods that can quantify proteins and small molecules, the enzyme of interest, co-enzymes, substrate, co-factors and metabolites can all be quantified in a single experiment. Extension of the idea to multiple enzyme pathways might produce a more complete picture of the larger system with less variability and noise than an integrative approach.
This presentation will highlight the collection of tools that make unified molecular analysis possible, illustrate the approach of chemoplexing with an example enzyme system (UGT enzymes), and discuss the current challenges associated with unified analysis and future opportunities for improvements in technology and methodology to support unified analysis of biological systems.
Carmen is President of PharmaCadence Analytical Services, LLC, a small consulting and laboratory group located in Hatfield, Pennsylvania. Prior to founding PharmaCadence, Carmen was a senior research scientist at Merck & Co., where she was a group leader, scientific mentor, and project team representative in the Department of Drug Metabolism over the course of more than 18 years. At Merck, Carmen was responsible for developing and applying mass spectrometric (LC-MS) and ligand binding analytical approaches to support discovery and development of small molecule and biologics drug candidates. As team leader, she collaborated in the design and implementation of departmental and cross-functional analytical strategies for the support of large molecule therapeutic modalities, including antibodies, proteins, peptides and siRNA. Under Carmen's guidance, analytical techniques including, mouse microsampling, miniaturization of ELISA, and general immunoassay formats were integrated into routine discovery PK of biologics. In addition, LC-MS/MS approaches for the quantitative analysis of proteins, PEGylated proteins and peptides in biological matrices were developed to support early discovery PK studies. Over the latter two years, she was responsible for the conduct of preclinical PK studies and other issue-driven studies to support discovery and preclinical development of biologics drug candidates. In 2009, she co-founded PharmaCadence Analytical Services, LLC to provide professional LC-MS-based analytical services to pharmaceutical and bio-medical researchers. She and her co-founder believe that analysis should never be the limiting factor in biological research, and strive to bring forward innovative LC-MS-based approaches to help assess biological systems, in the hopes of accelerating development of new medicines to improve the quality of life.
Carmen received her S.B. degree in Chemistry from the Massachusetts Institute of Technology, and obtained her Ph.D. in Chemistry from the University of Pennsylvania, under the direction of Dr. Barry S. Cooperman. The subject of her doctoral thesis is "Characterization of the Chloramphenicol Binding Site in E. coli Ribosomes". Her research interests cover a number of key areas in quantitative analysis through mass spectrometry including, separation sciences, protein chemistry, protein expression, and drug metabolism and pharmacokinetic (DMPK) studies.
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