October 2016 Meeting Announcement, Delaware Valley Mass Spectrometry Discussion Group
- Topic: "Discovery and Targeted Analysis of Whole Proteins and Their Complexes by Top Down Proteomics
- Speaker:Neil Kelleher, Northwestern University
- Date: Monday, October 10, 2016. 6:00 PM
- Please RSVP to firstname.lastname@example.org by Thursday October 6.
- Time: Social Hour: 6:00 PM.
Talk: 7:00 PM.
- Place: Department of Chemistry, Villanova University (Mendel Hall 154)
While top down mass spectrometry has become synonymous with the direct analysis of intact proteins via mass spectrometry, the term more generally denotes an approach to analytical measurement that recognizes the value of retaining as much information as possible about a system prior to its interrogation. By avoiding proteolytic digestion, proteoform-specific information can be uncovered in translational proteomics, and brief updates about progress will be shared at DVMSDG. However, the top down philosophy is equally applicable to the level of protein-protein and protein-ligand interactions, so some new approaches for native-TDPs will also be described for the analysis of protein complexes in an untargeted fashion. Providing information from intact complex mass to subunit backbone fragment ions, this approach enables identification and characterization of protein interactions. By preserving non-covalent interaction throughout sample preparation and ionization, top down mass spectrometry can operate at the next level of hierarchy in organization of proteins in biological systems.
Dr. Kelleher's laboratory has three main sub-groups working in the areas of Top Down Proteomics, Natural Products Biosynthesis/Discovery, and Cancer Epigenetics. The Kelleher group has been successful in driving both technology development and applications of high performance mass spectrometry at the interface of chemistry and biology. Since 2011, Dr. Kelleher has served as the director of the Proteomics Center of Excellence at Northwestern University, where dozens of Northwestern laboratories are supported and beyond state-of-the-art in Top Down proteomics is developed. Dr. Kelleher was elected Treasurer of the American Society for Mass Spectrometry in 2012 and established the Consortium for Top Down Proteomics that same year. In September 2012 Dr. Kelleher gave the keynote address at World HUPO (Human Proteome Organization), where he described a "Top Down" version of the Human Proteome Project viewable at this URL (http://www.kelleher.northwestern.edu/human-proteome-project/the-talk). With more than 200 papers published over the course of his career and teaching duties in two departments, Dr. Kelleher is a trans-disciplinary investigator with visible streaks of international impact in mass spectrometry-based proteomics and the discovery of new natural products from the microbial world. Validation of protein-based biomarkers in organ transplantation and cancers of the blood are among the focused areas currently being pursued in clinical research at Northwestern.
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