October 1999 Meeting Announcement, Delaware Valley Mass Spectrometry Discussion Group
- Topic: "Novel Mass-Based Approaches for Probing Viral Structure and
Performing Biomolecule Analysis"
- Speaker: Dr. Gary Siuzdak, Scripps Research Institute
- Date: Monday, October 11, 1999. 7:00 PM
- Time: Social Hour: 7:00 PM.
Talk: 7:30 PM.
- Place: Merck, West Point, 37 Auditorium
Viruses - Virus particles are stable yet exhibit highly dynamic
characteristics throughout their life cycle. Because these dynamic
properties can only be inferred from the X-ray structure, new methods that
are sensitive to viral capsid mobility must be developed. In the past, we
have found MALDI-MS with time resolved limited proteolysis to be a
sensitive indicator of viral capsid dynamics, and more recently we have
used LC/MS to further probe the effect of drugs on capsid mobility. These
experiments demonstrate that MALDI and LC/MS can be used to quantitatively
examine tryptic digestion rates (both inhibition and acceleration) by
measuring the product peptides as a function of drugs complexing the virus.
We have also designed a new class of drugs to inactivate viruses based on
DIOS - A new ionization strategy for biomolecular mass
spectrometry has been developed based on pulsed laser desorption/ionization
from porous silicon surfaces. Desorption/ionization on silicon (DIOS) uses
silicon to trap analytes deposited on the surface and laser radiation to
vaporize and ionize these molecules. DIOS is demonstrated for biomolecules
with little or no fragmentation, in contrast to what is typically observed
with other direct desorption/ionization approaches. The ability to perform
these measurements without a matrix also makes it more amenable to small
molecule analysis. The ease of chemically and structurally modifying
silicon has also been used to optimize the ionization characteristics of
the surface for biomolecular applications. Overall, desorption/ionization
on silicon permits analysis of a wide range of molecules with good
sensitivity and a potential for automation, as well as compatibility with
microfluidics and microchip technology on silicon.
Please send any comments, corrections, or suggestions to
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