High Resolution Mass Spectrometry

This experiment was developed by S.E. Van Bramer for Chemistry 465 at Widener University.

Introduction:

High resolution mass spectrometry may be used for unambiguous assignment of the molecular formula of a compound. This is extremely useful for verifying the identity of a synthesis product or identifying an unknown. However, the experiment requires very expensive and complex instrumentation. In this experiment we will be using a high reslution double sector mass spectrometer. Obtaining the highest resolution possible with this instrument requires a considerable amount of time and effort to tune the instrument. After the instrument is tuned, it is quite straightforward to obtain a series of high resolution spectra that will determine the m/z for the molecular ion to about 1 ppm (0.001 u for an ion at m/z 100).


Experimental:

  1. Overview of the instrument. Introduction and tuning of the instrument.

  2. Calibrate mass spectrometer. Introduction to calibration for high resolution experiment

  3. Data to collect. Adjust and tune instrument to obtain highest resolution possible.


Laboratory Write-up:

Your Laboratory write-up should include the following information
  1. (10 pts) An abstract for this experiment.

  2. (20 pts) Results section.

  3. (10 pts) Clearly labeled spectra spectra for each compound.

  4. (10 pts) The Carbon pages from your laboratory manual.


This page is maintained by
Scott Van Bramer
Department of Chemistry
Widener University
Chester, PA 19013

Please send any comments, corrections, or suggestions to svanbram@science.widener.edu.

This page has been accessed 2462 times since 1/5 /96 .
Last Updated Thursday, August 31, 2000 08:17:41