Laboratory Experiments


Lab Reports

  1. Scientific Writing
  2. Sample Abstracts
  3. Plagiarism
  4. Graphing Notes

Computer Simulations

Week 1, September 6
Mathcad Documents (Homework Assignment)


FTIR Experiments

Week 2, September 13.
FTIR Experiment. (50 pts 1st draft, due 1 week; 50 pts rewrite, due 1 week) In this experiment you will collect infrared spectra, optimize data acquisition, demonstrate signal averaging, and change other acquisition parameters.


Mass Spectrometry Experiments

Week 3, September 20.
Introduction to Mass Spectrometry Experiment (100 pts, due 2 weeks) In this experiment you will obtain mass spectra for a set of unknowns.

Week 4, September 27.
Interpretation of Mass Spectra. Work on data from "Introduction to Mass Spectrometry Experiment" and Unknowns.

Week 5, October 4
Low Energy Electron Ionization and Chemical Ionization Mass Spectrometry Experiment (100 pts, due 3 weeks) In the first part of this experiment you will study the effect of different ionization energies on the fragmentation patterns of several different compounds. This part of the experiment is based upon a journal article: Maccoll, A. Org. Mass Spectrom., 1986, 21, 601-611. Low Energy, Low Temperature Mass Spectra 6-A Synoptic View (See Reading Notes.) In the second part of this experiment you will obtain methane CI mass spectra to compared with the EI spectra. This experiment is based upon a journal article: Munson, M.S.B.; Field, F.H. J. Am. Chem. Soc., 1966, 88, 2621-2630. Chemical Ionization Mass Spectrometry. I. General Introduction. (See Reading Notes.)

Week 6, October 11
High Resolution Mass Spectrometry Experiment (50 pts, due 1 week) In this experiment you will acquire high resolution mass spectra of several compounds and use this data to determine the molecular formula for the compounds.
VG 70SE Operation
PFK Spectrum or Table
Exact Masses of the Elements from SIS
Exact Mass Calculator from SIS


NMR Experiments

Week 7, October 18
Simulations of the FID
Work through NMR Tutor Software

Week 8, October 25
Introduction to the NMR. (100 pts, due 3 weeks) In this experiment you will obtain and process a proton and a carbon NMR spectrum. 2-methyl-1-propanol and pamoic acid will be used for samples. The following handouts contain instructions for operating the instrument and for processing data. These handouts will be used for all NMR experiments.

  1. NMR Operations for QE 300 with Tecmag Data System
  2. Mac NMR
  3. Data Processing with Nuts (brief) (detailed)

Week 9, November 1
NMR Data Processing

Week 10, November 8
NMR Interpretation

Week 11, November 15
T1 Inversion Recovery Experiment (100 pts, due 2 weeks) In this experiment you will study the kinetics of the basic relaxation processes for NMR.
T2 Hahn Spin Echo Demo This experiment will demonstrate the spin echo experiment.

Week 12, November 29
2-D NMR Experiments (50 pts, due 2 weeks) This experiment is a look at different 2D NMR experiments frequently used to solve complex structural problems. This includes APT, DEPT, COSY, NOESY, and HETCOR.
2-D Interpretation.

Week 13, December 6
2-D Interpretation.

Week 15, December 13
Spectroscopic Identification of an Unknown. (100 pts, due 1 week) In this experiment you will receive an unknown organic compound. Using the spectroscopic techniques studied this semester (IR, MS, and NMR), identify your unknown and interpret the spectra. You may not use any spectral libraries or shift prediction software for this experiment.


Last Updated: 8/28/2007